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KMID : 0370220080520010007
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Yakhak Hoeji
2008 Volume.52 No. 1 p.7 ~ p.11
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Validation and Determination of Glycyrrhizic Acid as a Marker Substance in Bu-Zhong-Yi-Qi-Tang by HPLC/DAD
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Baik Joo-Hyun
Kim Sun-Min
Ahn Ji Won
Cho Chang-Hee
Oh Mi-Hyun
Cho Jung-Hee
Lee Mi-Kyeong
Kim Hyo-Jin
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Abstract
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A high performance liquid chromatographic (HPLC) method for the determination of glycyrrhizic acid was developed for the quality control of traditional herbal medicinal preparation Bu-Zhong-Yi-Qi-Tang (BZYQT), which is well-known herbal medicine used as tonic. RP-HPLC analysis was carried out using Capcell pak MG column and a mobile phase consisting of acetonitrile and water containing 0.03% phosphoric acid (pH 2.46) at a flow rate of 1.0 ml/min. The optimum wavelength for the detection of the glycyrrhizic acid was found at 250 nm using diode-array UV/VIS detector. The glycyrrhizic acid in BZYQT shows good linearity(r²>0.999) in the range of to 500 . The limit of detection (LOD) was less than 5 ng and R.S.D for intra-day and inter-day reproducibility was less than 7%. The mean recovery of the glycyrrhizic acid was . These results suggest that the developed HPLC method is simple and efficient, and could be contributed for the quality control of commercial BZYQT products.
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KEYWORD
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Bu-Zhong-Yi-Qi-Tang, Glycyrrhizic acid, HPLC, Quantification, Validation
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